Single-cell sequencing of immune cells from the coronary sinus reveals immune mechanisms of the progression of persistent atrial fibrillation
Chaofeng Chen,
Yang Pang,
Kuan Cheng,
Xiaofei Gao,
Yunlong Ling,
Ye Xu,
Jing Wu,
Yi-zhou Xu,
Qingxing Chen,
Wen-qing Zhu,
Jun-bo Ge
Affiliations
Chaofeng Chen
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China; Department of Cardiology, Hangzhou First People Hospital, 261 Huansha Road, Hangzhou 310000, China
Yang Pang
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China
Kuan Cheng
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China
Xiaofei Gao
Department of Cardiology, Hangzhou First People Hospital, 261 Huansha Road, Hangzhou 310000, China
Yunlong Ling
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China
Ye Xu
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China
Jing Wu
Department of Cardiology, Hangzhou First People Hospital, 261 Huansha Road, Hangzhou 310000, China
Yi-zhou Xu
Department of Cardiology, Hangzhou First People Hospital, 261 Huansha Road, Hangzhou 310000, China
Qingxing Chen
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China; The Second People's Hospital of Kashi, Xinjiang, China; Corresponding author
Wen-qing Zhu
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China; Corresponding author
Jun-bo Ge
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200000, China
Summary: Identifying the atlas of immune cells from coronary sinus circulation (CSC) of patients with persistent atrial fibrillation (PerAF) may provide new insights into the role of immune cells in the progression of AF. Single-cell sequencing revealed substantial alterations in immune cells from CSCs of patients with PerAF, especially a markedly elevated abundance of T cells, after which we identified a T cell subset: FGFBP2(+)TRDC(−)CD4(−) T cells (Ftc-T cells), which can promote the proliferation of cardiac fibroblasts (CFs),and the proportion of Ftc-T had a positive linear with AF recurrence post catheter ablation (CA). Moreover, IFI27 was found to be highly enriched in Ftc-T cells and promoted CFs proliferation and collagen expression. Altogether, our findings represent a unique resource providing in-depth insights into the heterogeneity of the immune cell from CSC of patients with PerAF and highlight the potential role of Ftc-T cells and IFI27 for AF progression.
