The effect of PEGylated iron oxide nanoparticles on sheep ovarian tissue: An ex-vivo nanosafety study
Sareh Karimi,
Seyed Nasrollah Tabatabaei,
Arno C. Gutleb,
Marefat Ghaffari Novin,
Alireza Ebrahimzadeh-Bideskan,
Zahra Shams Mofarahe
Affiliations
Sareh Karimi
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Seyed Nasrollah Tabatabaei
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Pediatrics, Physiology and Pharmacology, University of Montreal, Montreal, QC, Canada
Arno C. Gutleb
Department of Environmental Research and Innovation (ERIN), Luxembourg Institute of Science and Technology (LIST), Esch s/Alzette, Luxembourg
Marefat Ghaffari Novin
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Alireza Ebrahimzadeh-Bideskan
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Iran; Corresponding author.
Zahra Shams Mofarahe
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author.
Today, nanotechnology plays an important role in our ever-continuous quest to improve the quality of human life. Because of their infinitesimal size, nanostructures can actively interact and alter cellular functions. Therefore, while the clinical benefits of nanotechnology may outweigh most of the associated risks, assessment of the cytotoxicity of nanostructures in respect to cells and tissues early in product development processes is of great significance. To the best of our knowledge, no such assessment has been performed for nanomaterials on the ovarian cortex before. Herein, silica-coated, PEGylated silica-coated, and uncoated iron oxide nanoparticles (IONP) with core diameter of 11 nm (±4.2 nm) were synthesized. The oxidative stress in cultured ovarian tissue exposed to the various IONP was subsequently assessed. The results indicate that among the four groups, uncoated IONP induce the most oxidative stress on the ovarian cortex while tissues treated with PEGylated IONP exhibit no significant change in oxidative stress.
