Drug Design, Development and Therapy (Nov 2023)

Panax quinquefolium L. and Salvia miltiorrhiza Bunge. Enhances Angiogenesis by Regulating the miR-155-5p/HIF-1α/VEGF Axis in Acute Myocardial Infarction

  • Li X,
  • Liu R,
  • Liu W,
  • Liu X,
  • Fan Z,
  • Cui J,
  • Wu Y,
  • Yin H,
  • Lin Q

Journal volume & issue
Vol. Volume 17
pp. 3249 – 3267

Abstract

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Xingxing Li,1 Rongpeng Liu,1 Wei Liu,1 Xin Liu,2 Zongjing Fan,1 Jie Cui,1 Yang Wu,1 Huijun Yin,3 Quan Lin1 1Dongfang Hospital, Beijing University of Chinese Medicine, Beijing, 100078, People’s Republic of China; 2The Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, 100029, People’s Republic of China; 3Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, People’s Republic of ChinaCorrespondence: Quan Lin, Dongfang Hospital, Beijing University of Chinese Medicine, No. 6, Fangxingyuan District 1, Fengtai District, Beijing, 100078, People’s Republic of China, Tel +86-10-67689757, Email [email protected]: Combination of Panax quinquefolium L and Salvia miltiorrhiza Bunge. (PS) has been widely used in the clinical treatment of ischemic heart disease. The purpose of this study was to explore the therapeutic effect and mechanism of PS on angiogenesis in rats after acute myocardial infarction (AMI).Methods: A rat model of AMI was established by ligating the left anterior descending (LAD) artery. The grouping and administration scheme were as follows: sham group, model group, PS low-dose (PS-L) group, PS high-dose (PS-H) group, PX-478 group and angiotensin converting enzyme inhibitor (ACEI) group. After 28 days of treatment, echocardiography, myocardial infarct size, some angiogenesis markers and the miR-155-5p/HIF-1α/VEGF axis were measured.Results: PS improved cardiac structure and function, reduced infarct size, and alleviated myocardial fibrosis and inflammatory cell infiltration in AMI rats. Mechanistically, PS enhanced the expression of HGF and bFGF in serum, increased the levels of MVD and CD31 in myocardial tissues, and inhibited the activation of the miR-155-5p/HIF-1α/VEGF pathway, which ultimately promoted angiogenesis. In addition, the regulatory effect of PS on angiogenesis was partly abolished by PX-478.Conclusion: PS increased the expression of MVD and CD31 in the myocardium and stimulated angiogenesis. The above effects of PS may be associated with the inhibition of the miR-155-5p/HIF-1α/VEGF axis. Keywords: Panax quinquefolium L, Salvia miltiorrhiza Bunge, angiogenesis, acute myocardial infarction, miR-155-5p/HIF-1α/VEGF

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