Analysis region of intron 5 sequence of PIT-1 gene in broiler chickens Arian line

Abstract

Sequencing and analysis of the data obtained are best and most common methods in studies of genetic diversity. PIT-1gene is known as growth hormone IGF-1 gene .this gene is a member of family of transcription factors in the poultry is located on chromosome 1, with a length of approximately 14 kb. This gene has seven exons and five introns in poultry. The purpose of this study was analysis region of intron 5 sequence of PIT-1 gene in broiler chickens Arian lines and determining its phylogenetic relationship with other chicken breeds. For this study, blood samples were collected of seven broiler chickens of both sex Arian lines. After extracting DNA from them, target gene was amplified with specific primers and after purification was sequenced. Five different haplotypes were determined based on six single nucleotide polymorphism sequence and the final sequences of each haplotype with length approximate 260 bp which includes 21/92 % adenine, 18/8 % guanine, 32/69 % cytosine and 27/31 % thymine. After ensuring the accuracy of sequencing, submitted to gene bank database (NCBI) with accession number of JQ946630- JQ946636.The phylogenic tree was drawn with consensus sequence of PIT-1 gene of Arian line chicken and other similar sequences of different chicken breeds obtained from gene bank. Results the phylogeny indicated that Arian line chicken and American Chickens are on a lineages

Keywords

• phylogeny
• pit-1 gene
• broiler chickens
• sequencing