Nature Communications (Oct 2018)

Characterization of a long overlooked copper protein from methane- and ammonia-oxidizing bacteria

  • Oriana S. Fisher,
  • Grace E. Kenney,
  • Matthew O. Ross,
  • Soo Y. Ro,
  • Betelehem E. Lemma,
  • Sharon Batelu,
  • Paul M. Thomas,
  • Victoria C. Sosnowski,
  • Caroline J. DeHart,
  • Neil L. Kelleher,
  • Timothy L. Stemmler,
  • Brian M. Hoffman,
  • Amy C. Rosenzweig

DOI
https://doi.org/10.1038/s41467-018-06681-5
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 12

Abstract

Read online

Methane- and ammonia-oxidizing bacteria use the integral membrane, copper-dependent enzymes particulate methane monooxygenase (pMMO) and ammonia monooxygenase (AMO) to oxidize methane and ammonia. Here the authors structurally characterize the copper-binding protein PmoD, which contains an unusual CuA site and their genetic analyses strongly support a pMMO and AMO related function of PmoD.