Mutation rate and spectrum in obligately outcrossing <i>Caenorhabditis elegans</i> mutation accumulation lines subjected to RNAi-induced knockdown of the mismatch repair gene <i>msh-2</i>

Abstract

AbstractDNA mismatch repair (MMR), an evolutionarily conserved repair pathway shared by prokaryotic and eukaryotic species alike, influences molecular evolution by detecting and correcting mismatches, thereby protecting genetic fidelity, reducing the mutational load, and preventing lethality. Herein we conduct the first genome-wide evaluation of the alterations to the mutation rate and spectrum under impaired activity of the MutSαmsh-2Caenorhabditis elegansfog-2(lf)msh-2msh-2C. elegansmsh-2