Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells
Anna S. Efimova,
Mariya A. Ustimova,
Nelly S. Chmelyuk,
Maxim A. Abakumov,
Yury V. Fedorov,
Olga A. Fedorova
Affiliations
Anna S. Efimova
Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119334 Moscow, Russia
Mariya A. Ustimova
Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119334 Moscow, Russia
Nelly S. Chmelyuk
Department of Medical Nanobiotechnoilogy, Pirogov Russian National Research Medical University, Ostrovityanova Str. 1, 117997 Moscow, Russia
Maxim A. Abakumov
Department of Medical Nanobiotechnoilogy, Pirogov Russian National Research Medical University, Ostrovityanova Str. 1, 117997 Moscow, Russia
Yury V. Fedorov
Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119334 Moscow, Russia
Olga A. Fedorova
Laboratory of Photoactive Supramolecular Systems, A. N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences, Vavilova Str. 28, 119334 Moscow, Russia
New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influenced their binding modes, binding affinities, and spectroscopic responses. Biological experiments showed the low toxicity of the obtained dyes and their applicability as selective dyes for mitochondria in living cells.
