Nature Communications (Sep 2018)
14-3-3 proteins activate Pseudomonas exotoxins-S and -T by chaperoning a hydrophobic surface
- Tobias Karlberg,
- Peter Hornyak,
- Ana Filipa Pinto,
- Stefina Milanova,
- Mahsa Ebrahimi,
- Mikael Lindberg,
- Nikolai Püllen,
- Axel Nordström,
- Elinor Löverli,
- Rémi Caraballo,
- Emily V. Wong,
- Katja Näreoja,
- Ann-Gerd Thorsell,
- Mikael Elofsson,
- Enrique M. De La Cruz,
- Camilla Björkegren,
- Herwig Schüler
Affiliations
- Tobias Karlberg
- Department of Biosciences and Nutrition, Karolinska Institutet
- Peter Hornyak
- Department of Biosciences and Nutrition, Karolinska Institutet
- Ana Filipa Pinto
- Department of Biosciences and Nutrition, Karolinska Institutet
- Stefina Milanova
- Department of Cellular and Molecular Biology, Karolinska Institutet
- Mahsa Ebrahimi
- Department of Biosciences and Nutrition, Karolinska Institutet
- Mikael Lindberg
- Protein Expertise Platform, Umeå University
- Nikolai Püllen
- Department of Biosciences and Nutrition, Karolinska Institutet
- Axel Nordström
- Department of Biosciences and Nutrition, Karolinska Institutet
- Elinor Löverli
- Department of Biosciences and Nutrition, Karolinska Institutet
- Rémi Caraballo
- Department of Chemistry, Umeå University
- Emily V. Wong
- Molecular Biophysics and Biochemistry, Yale University
- Katja Näreoja
- Department of Biosciences and Nutrition, Karolinska Institutet
- Ann-Gerd Thorsell
- Department of Biosciences and Nutrition, Karolinska Institutet
- Mikael Elofsson
- Department of Chemistry, Umeå University
- Enrique M. De La Cruz
- Molecular Biophysics and Biochemistry, Yale University
- Camilla Björkegren
- Department of Biosciences and Nutrition, Karolinska Institutet
- Herwig Schüler
- Department of Biosciences and Nutrition, Karolinska Institutet
- DOI
- https://doi.org/10.1038/s41467-018-06194-1
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 11
Abstract
The cellular toxicity of Pseudomonas exotoxin-S and -T depends on their activation by 14-3-3 but the underlying molecular mechanism is not fully understood. Here, the authors show that a previously unrecognized 14-3-3:exotoxin binding interface is sufficient for complex formation and toxin activation.
