Effects of maternal undernutrition during late pregnancy on the regulatory factors involved in growth and development in ovine fetal perirenal brown adipose tissue

Huan Yang; Chi Ma; Yang Zi; Min Zhang; Yingchun Liu; Kaifeng Wu; Feng Gao

doi:10.5713/ab.21.0199

Animal Bioscience (Jul 2022)

Effects of maternal undernutrition during late pregnancy on the regulatory factors involved in growth and development in ovine fetal perirenal brown adipose tissue

Huan Yang,
Chi Ma,
Yang Zi,
Min Zhang,
Yingchun Liu,
Kaifeng Wu,
Feng Gao

Affiliations

Huan Yang: College of Animal Science, Inner Mongolia Key Laboratory of animal nutrition and feed, Inner Mongolia Agricultural University, Hohhot, 010018, China
Chi Ma: College of Animal Science, Inner Mongolia Key Laboratory of animal nutrition and feed, Inner Mongolia Agricultural University, Hohhot, 010018, China
Yang Zi: College of Animal Science, Inner Mongolia Key Laboratory of animal nutrition and feed, Inner Mongolia Agricultural University, Hohhot, 010018, China
Min Zhang: College of Animal Science, Inner Mongolia Key Laboratory of animal nutrition and feed, Inner Mongolia Agricultural University, Hohhot, 010018, China
Yingchun Liu: College of Life Science, Inner Mongolia Key Laboratory of Biomanufacturing, Inner Mongolia Agricultural University, Hohhot, 010018, China
Kaifeng Wu: College of Life Science, Inner Mongolia Key Laboratory of Biomanufacturing, Inner Mongolia Agricultural University, Hohhot, 010018, China
Feng Gao: College of Animal Science, Inner Mongolia Key Laboratory of animal nutrition and feed, Inner Mongolia Agricultural University, Hohhot, 010018, China

DOI: https://doi.org/10.5713/ab.21.0199
Journal volume & issue: Vol. 35, no. 7
pp. 1010 – 1020

Abstract

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Objective The experiment was conducted to evaluate the effects of maternal undernutrition during late pregnancy on the expressions of genes involved in growth and development in ovine fetal perirenal brown adipose tissue (BAT). Methods Eighteen ewes with singleton fetuses were allocated to three groups at day 90 of pregnancy: restricted group 1 (RG1, 0.33 MJ metabolisable energy [ME]/kg body weight [BW]0.75/d, n = 6), restricted group 2 (RG2, 0.18 MJ ME/kg BW0.75/d, n = 6), and a control group (CG, ad libitum, 0.67 MJ ME/kg BW0.75/d, n = 6). The fetuses were removed at day 140 of pregnancy. All data were analyzed by using the analysis of variance procedure. Results The perirenal fat weight (p = 0.0077) and perirenal fat growth rate (p = 0.0074) were reduced in RG2 compared to CG. In fetal perirenal BAT, the protein level of uncoupling protein 1 (UCP1) (p = 0.0001) was lower in RG1 and RG2 compared with CG and UCP1 mRNA expression (p = 0.0265) was decreased in RG2. The protein level of myogenic factor 5 (Myf5) was also decreased in RG2 (p = 0.0001). In addition, mRNA expressions of CyclinA (p = 0.0109), CyclinB (p = 0.0019), CyclinD (p = 0.0015), cyclin-dependent kinase 1 (CDK1) (p = 0.0001), E2F transcription factor 1 (E2F1) (p = 0.0323), E2F4 (p = 0.0101), and E2F5 (p = 0.0018) were lower in RG1 and RG2. There were decreased protein expression of peroxisome proliferator-activated receptor-γ (PPARγ) (p = 0.0043) and mRNA expression of CCAAT/enhancer-binding protein-α (C/EBPα) (p = 0.0307) in RG2 and decreased PPARγ mRNA expression (p = 0.0008) and C/EBPα protein expression (p = 0.0015) in both RG2 and RG1. Furthermore, mRNA expression of bone morphogenetic protein 4 (BMP4) (p = 0.0083) and BMP7 (p = 0.0330) decreased in RG2 and peroxisome proliferator-activated receptor co-activator-1α (PGC-1α) reduced in RG2 and RG1. Conclusion Our observations support that repression of regulatory factors promoting differentiation and development results in the inhibition of BAT maturation in fetal perirenal fat during late pregnancy with maternal undernutrition.

Published in Animal Bioscience

ISSN: 2765-0189 (Print); 2765-0235 (Online)
Publisher: Asian-Australasian Association of Animal Production Societies
Country of publisher: Korea, Republic of
LCC subjects: Science: Zoology
Website: https://www.animbiosci.org/index.php

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