The study of factors affecting the efficiency of somatic transgenesis in chickens

Abstract

In this article we studied the impact of the use of retroviral vectors for genetic transformation of chicken oviduct cells in vivo to produce transgenic chickens bioreactors and investigated factors affecting the efficiency of local transgenesis in chickens. By using of retroviral vector system it was shown that with the efficiency of chickens oviduct cells transformation was at least 17,2 ± 3,1%.

Keywords

• куры
• трансгенез
• яйцевод
• биореакторы
• рекомбинантная ДНК