In vitro efficacy of Elephantorrhiza elephantina root extracts against adult Paramphistomum cervi in goats

Abstract

Elephantorrhiza elephantina is a medicinally important plant whose roots are used to control gastrointestinal parasites in goats. The use of plant-based anthelmintics as potential alternatives to synthetic anthelmintics in controlling gastrointestinal worms in ruminants is a promising area of research. The study was carried out to ascertain the efficacy of E. elephantina in controlling goat trematodes. Standard procedures were used to quantify the phytochemicals in the water, ethanol and methanol root extracts of E. elephantina. The quantitative phytochemical analysis revealed that alkaloids (93.24 ± 1.68%), condensed tannins (450.52 ± 0.15 mg CE/g), flavonoids (803.93 ± 0.13 mg QE/g), phenols (476.11 ± 0.37 mg GAE/g) and saponins (83.28 ± 1.72%) were present in E. elephantina root extracts. Paramphistomum cervi adult worms recovered from the rumen of freshly slaughtered goats were used. In vitro screening of ethanol, methanol and water extracts of E. elephantina for potential anthelmintic activity against adult P. cervi worm motility showed time and dose-dependent significant effects. Ethanol extracts showed a highly significant (P < 0.05) inhibition of motility (86.67 and 96.67%) at concentrations 7.5 and 15 mg/mL respectively, after 12 h of treatment, while methanol and water extracts exhibited a significant (P < 0.05) inhibition of motility (96.67 and 66.67% respectively) at concentration of 15 mg/mL 16 h post-exposure. Our findings confirm the efficacy of E. elephantina in controlling goat trematodes.

Keywords

• Anthelmintic activity
• Ethnoveterinary medicine
• Medicinal plants
• Gastrointestinal parasites
• Phytochemicals
• Structural changes