Communications Biology (Apr 2023)
Divalent nanobodies to platelet CLEC-2 can serve as agonists or antagonists
- Joanne C. Clark,
- Eleyna M. Martin,
- Luis A. Morán,
- Ying Di,
- Xueqing Wang,
- Malou Zuidscherwoude,
- Helena C. Brown,
- Deirdre M. Kavanagh,
- Johan Hummert,
- Johannes A. Eble,
- Bernhard Nieswandt,
- David Stegner,
- Alice Y. Pollitt,
- Dirk-Peter Herten,
- Michael G. Tomlinson,
- Angel García,
- Steve P. Watson
Affiliations
- Joanne C. Clark
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Eleyna M. Martin
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Luis A. Morán
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Ying Di
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Xueqing Wang
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Malou Zuidscherwoude
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Helena C. Brown
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Deirdre M. Kavanagh
- Department of Biochemistry, University of Oxford
- Johan Hummert
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Johannes A. Eble
- Institute for Physiological Chemistry & Pathobiochemistry, University of Münster
- Bernhard Nieswandt
- Institute of Experimental Biomedicine I, University Hospital and Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Würzburg
- David Stegner
- Institute of Experimental Biomedicine I, University Hospital and Rudolf Virchow Center for Integrative and Translational Bioimaging, University of Würzburg
- Alice Y. Pollitt
- Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading
- Dirk-Peter Herten
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- Michael G. Tomlinson
- Centre of Membrane Proteins and Receptors (COMPARE), The Universities of Birmingham and Nottingham
- Angel García
- Centre for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela, and Instituto de Investigación Sanitaria de Santiago (IDIS)
- Steve P. Watson
- Institute of Cardiovascular Sciences, Level 1 IBR, College of Medical and Dental Sciences, University of Birmingham
- DOI
- https://doi.org/10.1038/s42003-023-04766-6
- Journal volume & issue
-
Vol. 6,
no. 1
pp. 1 – 17
Abstract
Fluorescence correlation spectroscopy on crosslinked nanobodies tested on C-type lectin-like receptor 2 (CLEC-2) show different outcomes for divalent and tetravalent nanobody ligands on CLEC-2 clustering, also dependent on CLEC-2 expression levels.
