Clinical, Cosmetic and Investigational Dermatology (Jan 2022)
Research on Correlations of miR-196a Expression with Progression and Prognosis of Cutaneous Squamous Cell Carcinoma
Abstract
Wei Zhang,1 Xiaowen Yang,2 Jianping Lv,1 Shichang An3 1Department of Cosmetic Plastic Surgery, Weifang People’s Hospital, Weifang, Shandong, 261000, People’s Republic of China; 2Department of Stomatology, The People’s Hospital of Zhaoyuan City, Yantai, Shandong, 265400, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, Weifang People’s Hospital, Weifang, Shandong, 261000, People’s Republic of ChinaCorrespondence: Shichang AnDepartment of Oral and Maxillofacial Surgery, Weifang People’s Hospital, 151 Guangwen Street, Weifang, Shandong, 261000, People’s Republic of China, Tel +86-0536-8192557, Email [email protected]: This study aimed to investigate the correlation between miR-196a expression and the progression and prognosis of cutaneous squamous cell carcinoma (CSCC).Patients and Methods: Tissue samples and corresponding paracancerous tissue samples from 117 patients with CSCC were collected. The qRT-PCR analysis was used to detect the expression levels of miR-196a. Kaplan–Meier curve and Cox regression analyses were used to analyze the relationship between miR-196a expression and patients’ prognosis. The CCK-8 and transwell assays were used to explore the effects of miR-196a on the abilities of cell proliferation, migration, or invasion.Results: miR-196a expression was significantly up-regulated in CSCC tissues or cell lines, compared with adjacent normal tissues or cell lines, respectively. High expression of miR-196a was associated with positive lymph node metastasis, high TNM stages, and a lower five-year survival rate. The expression level of miR-196a was up-regulated and the proliferation, migration or invasion ability of cells were significantly increased accordingly.Conclusion: miR-196a is highly expressed in CSCC, thus affecting the occurrence and development of CSCC. More importantly, miR-196a was shown to have potential as a prognostic marker for CSCC.Keywords: miR-196a, progression, prognosis, cutaneous squamous cell carcinoma
