Elevated BTG2 improves the radiosensitivity of non‐small cell lung cancer (NSCLC) through apoptosis

Abstract

Abstract Background To identify radio‐responsive genes and explore the biological function of encoded proteins in non‐small cell lung cancer (NSCLC). Methods Radio‐responsive genes in irradiated H460 cells were screened from microarray data deposited in the Gene Expression Omnibus (GEO) database. A quantitative real time polymerase chain reaction assay was used to detect the expression of candidate radio‐responsive genes in irradiated cells. CCK‐8 assay, EDU assay, clone formation assay, immunofluorescence and flow cytometry were conducted to evaluate the biological function of B cell translocation gene 2 (BTG2) in NSCLC. Results Bioinformatic analysis using GES20549 showed that BTG2 was a radio‐responsive gene in irradiated H460 cells. The mRNA expression level of BTG2 was lower in H460 cells compared with that in BEAS‐2B normal lung epithelial cells. BTG2 expression was elevated upon IR exposure, in a dose‐dependent but not a time‐dependent manner. CCK‐8 and EDU assays revealed that BTG2 overexpression inhibited the growth rate of irradiated cells. Clone formation showed that elevated BTG2 promoted DNA damage of irradiated H460 cells. The number of γ‐H2AX foci induced by DNA damage was also markedly increased upon BTG2 overexpression. Flow cytometry showed that BTG2 increased IR‐induced cell apoptosis. Conclusions BTG2 may be a novel radio‐responsive factor and a promising therapeutic target for radiotherapy of NSCLC.

Keywords

• bioinformatics
• biological function
• BTG2
• H460 cells
• radio‐responsive genes