Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis

Eszter Szabó; Dóra Reé; Bálint Jezsó; Katalin Vincze; Gábor Földes; Andrea Á. Molnár; János M. Réthelyi; Ágota Apáti

Stem Cell Research (Dec 2020)

Generation of iPSC lines from peripheral blood mononuclear cells of identical twins both suffering from type 2 diabetes mellitus and one of them additionally diagnosed with atherosclerosis

Eszter Szabó,
Dóra Reé,
Bálint Jezsó,
Katalin Vincze,
Gábor Földes,
Andrea Á. Molnár,
János M. Réthelyi,
Ágota Apáti

Affiliations

Eszter Szabó: Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary
Dóra Reé: Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary
Bálint Jezsó: Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary
Katalin Vincze: Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary; Molecular Psychiatry Research Group, National Brain Research Program (NAP), Hungarian Academy of Sciences and Semmelweis University, Hungary
Gábor Földes: Heart and Vascular Center, Semmelweis University, Budapest, Hungary
Andrea Á. Molnár: Heart and Vascular Center, Semmelweis University, Budapest, Hungary
János M. Réthelyi: Molecular Psychiatry Research Group, National Brain Research Program (NAP), Hungarian Academy of Sciences and Semmelweis University, Hungary; Department of Psychiatry and Psychotherapy, Semmelweis University, Budapest, Hungary
Ágota Apáti: Institute of Enzymology, Research Center for Natural Sciences, Eötvös Loránd Research Network, Hungary; Corresponding author.

Journal volume & issue: Vol. 49
p. 102051

Abstract

Read online

Here we describe the generation of induced pluripotent stem cell (iPSC) lines from peripheral blood samples of identical twin sisters with type 2 diabetes mellitus (DM2). Two clonal lines from each patient (HU-DM2-A-1, HU-DM2-A-2 and HU-DM2-B-1, HU-DM2-B-2) were established via Sendai viral reprograming of peripheral blood mononuclear cells, and characterized to confirm pluripotency and genetic integrity. The established iPSC lines can help to investigate DM2 related cellular phenotypes and provide a model system for drug testing.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal