Preparation of Human Kidney Progenitor Cultures and Their Differentiation into Podocytes
Maria Elena Melica,
Maria Lucia Angelotti,
Giulia Antonelli,
Anna Peired,
Carolina Conte,
Letizia De Chiara,
Benedetta Mazzinghi,
Elena Lazzeri,
Laura Lasagni,
Paola Romagnani
Affiliations
Maria Elena Melica
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy
Maria Lucia Angelotti
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy
Giulia Antonelli
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy, Nephrology and Dialysis Unit, Meyer Children’s Hospital IRCCS, Florence 50139, Italy
Anna Peired
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), Univerity of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy
Carolina Conte
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy, Nephrology and Dialysis Unit, Meyer Children’s Hospital IRCCS, Florence 50139, Italy
Letizia De Chiara
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy
Benedetta Mazzinghi
Nephrology and Dialysis Unit, Meyer Children’s Hospital IRCCS, Florence 50139, Italy
Elena Lazzeri
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy
Laura Lasagni
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy
Paola Romagnani
Excellence Centre for Research, Transfer and High Education for the development of DE NOVO Therapies (DENOTHE), University of Florence, Florence 50139, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence 50139, Italy, Nephrology and Dialysis Unit, Meyer Children’s Hospital IRCCS, Florence 50139, Italy
Kidney diseases are a global health concern. Modeling of kidney disease for translational research is often challenging because of species specificities or the postmitotic status of kidney epithelial cells that make primary cultures, for example podocytes. Here, we report a protocol for preparing primary cultures of podocytes based on the isolation and in vitro propagation of immature kidney progenitor cells subsequently differentiated into mature podocytes. This protocol can be useful for studying physiology and pathophysiology of human kidney progenitors and to obtain differentiated podocytes for modeling podocytopathies and other kidney disorders involving podocytes.Graphical overview
