Different Effects of Phototherapy for Rat Glioma during Sleep and Wakefulness
Alexander Shirokov,
Inna Blokhina,
Ivan Fedosov,
Egor Ilyukov,
Andrey Terskov,
Dmitry Myagkov,
Dmitry Tuktarov,
Maria Tzoy,
Viktoria Adushkina,
Daria Zlatogosrkaya,
Arina Evsyukova,
Valeria Telnova,
Alexander Dubrovsky,
Alexander Dmitrenko,
Maria Manzhaeva,
Valeria Krupnova,
Matvey Tuzhilkin,
Inna Elezarova,
Nikita Navolokin,
Elena Saranceva,
Tatyana Iskra,
Ekaterina Lykova,
Oxana Semyachkina-Glushkovskaya
Affiliations
Alexander Shirokov
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia
Inna Blokhina
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Ivan Fedosov
Physics Department, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Egor Ilyukov
Physics Department, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Andrey Terskov
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Dmitry Myagkov
Physics Department, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Dmitry Tuktarov
Physics Department, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Maria Tzoy
Physics Department, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Viktoria Adushkina
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Daria Zlatogosrkaya
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Arina Evsyukova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Valeria Telnova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Alexander Dubrovsky
Physics Department, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Alexander Dmitrenko
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Maria Manzhaeva
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Valeria Krupnova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Matvey Tuzhilkin
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Inna Elezarova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Nikita Navolokin
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Elena Saranceva
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Tatyana Iskra
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Ekaterina Lykova
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
Oxana Semyachkina-Glushkovskaya
Department of Biology, Saratov State University, Astrakhanskaya Str. 83, 410012 Saratov, Russia
There is an association between sleep quality and glioma-specific outcomes, including survival. The critical role of sleep in survival among subjects with glioma may be due to sleep-induced activation of brain drainage (BD), that is dramatically suppressed in subjects with glioma. Emerging evidence demonstrates that photobiomodulation (PBM) is an effective technology for both the stimulation of BD and as an add-on therapy for glioma. Emerging evidence suggests that PBM during sleep stimulates BD more strongly than when awake. In this study on male Wistar rats, we clearly demonstrate that the PBM course during sleep vs. when awake more effectively suppresses glioma growth and increases survival compared with the control. The study of the mechanisms of this phenomenon revealed stronger effects of the PBM course in sleeping vs. awake rats on the stimulation of BD and an immune response against glioma, including an increase in the number of CD8+ in glioma cells, activation of apoptosis, and blockage of the proliferation of glioma cells. Our new technology for sleep-phototherapy opens a new strategy to improve the quality of medical care for patients with brain cancer, using promising smart-sleep and non-invasive approaches of glioma treatment.
