Nature Communications (Oct 2018)
Megahertz serial crystallography
- Max O. Wiedorn,
- Dominik Oberthür,
- Richard Bean,
- Robin Schubert,
- Nadine Werner,
- Brian Abbey,
- Martin Aepfelbacher,
- Luigi Adriano,
- Aschkan Allahgholi,
- Nasser Al-Qudami,
- Jakob Andreasson,
- Steve Aplin,
- Salah Awel,
- Kartik Ayyer,
- Saša Bajt,
- Imrich Barák,
- Sadia Bari,
- Johan Bielecki,
- Sabine Botha,
- Djelloul Boukhelef,
- Wolfgang Brehm,
- Sandor Brockhauser,
- Igor Cheviakov,
- Matthew A. Coleman,
- Francisco Cruz-Mazo,
- Cyril Danilevski,
- Connie Darmanin,
- R. Bruce Doak,
- Martin Domaracky,
- Katerina Dörner,
- Yang Du,
- Hans Fangohr,
- Holger Fleckenstein,
- Matthias Frank,
- Petra Fromme,
- Alfonso M. Gañán-Calvo,
- Yaroslav Gevorkov,
- Klaus Giewekemeyer,
- Helen Mary Ginn,
- Heinz Graafsma,
- Rita Graceffa,
- Dominic Greiffenberg,
- Lars Gumprecht,
- Peter Göttlicher,
- Janos Hajdu,
- Steffen Hauf,
- Michael Heymann,
- Susannah Holmes,
- Daniel A. Horke,
- Mark S. Hunter,
- Siegfried Imlau,
- Alexander Kaukher,
- Yoonhee Kim,
- Alexander Klyuev,
- Juraj Knoška,
- Bostjan Kobe,
- Manuela Kuhn,
- Christopher Kupitz,
- Jochen Küpper,
- Janine Mia Lahey-Rudolph,
- Torsten Laurus,
- Karoline Le Cong,
- Romain Letrun,
- P. Lourdu Xavier,
- Luis Maia,
- Filipe R. N. C. Maia,
- Valerio Mariani,
- Marc Messerschmidt,
- Markus Metz,
- Davide Mezza,
- Thomas Michelat,
- Grant Mills,
- Diana C. F. Monteiro,
- Andrew Morgan,
- Kerstin Mühlig,
- Anna Munke,
- Astrid Münnich,
- Julia Nette,
- Keith A. Nugent,
- Theresa Nuguid,
- Allen M. Orville,
- Suraj Pandey,
- Gisel Pena,
- Pablo Villanueva-Perez,
- Jennifer Poehlsen,
- Gianpietro Previtali,
- Lars Redecke,
- Winnie Maria Riekehr,
- Holger Rohde,
- Adam Round,
- Tatiana Safenreiter,
- Iosifina Sarrou,
- Tokushi Sato,
- Marius Schmidt,
- Bernd Schmitt,
- Robert Schönherr,
- Joachim Schulz,
- Jonas A. Sellberg,
- M. Marvin Seibert,
- Carolin Seuring,
- Megan L. Shelby,
- Robert L. Shoeman,
- Marcin Sikorski,
- Alessandro Silenzi,
- Claudiu A. Stan,
- Xintian Shi,
- Stephan Stern,
- Jola Sztuk-Dambietz,
- Janusz Szuba,
- Aleksandra Tolstikova,
- Martin Trebbin,
- Ulrich Trunk,
- Patrik Vagovic,
- Thomas Ve,
- Britta Weinhausen,
- Thomas A. White,
- Krzysztof Wrona,
- Chen Xu,
- Oleksandr Yefanov,
- Nadia Zatsepin,
- Jiaguo Zhang,
- Markus Perbandt,
- Adrian P. Mancuso,
- Christian Betzel,
- Henry Chapman,
- Anton Barty
Affiliations
- Max O. Wiedorn
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Dominik Oberthür
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Richard Bean
- European XFEL GmbH
- Robin Schubert
- The Hamburg Center for Ultrafast Imaging, Universität Hamburg
- Nadine Werner
- Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, Universität Hamburg
- Brian Abbey
- Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University
- Martin Aepfelbacher
- Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE)
- Luigi Adriano
- Deutsches Elektronen-Synchrotron DESY
- Aschkan Allahgholi
- Deutsches Elektronen-Synchrotron DESY
- Nasser Al-Qudami
- European XFEL GmbH
- Jakob Andreasson
- Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University
- Steve Aplin
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Salah Awel
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Kartik Ayyer
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Saša Bajt
- Deutsches Elektronen-Synchrotron DESY
- Imrich Barák
- Institute of Molecular Biology, SAS
- Sadia Bari
- Deutsches Elektronen-Synchrotron DESY
- Johan Bielecki
- European XFEL GmbH
- Sabine Botha
- The Hamburg Center for Ultrafast Imaging, Universität Hamburg
- Djelloul Boukhelef
- European XFEL GmbH
- Wolfgang Brehm
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Sandor Brockhauser
- European XFEL GmbH
- Igor Cheviakov
- Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE)
- Matthew A. Coleman
- Lawrence Livermore National Laboratory
- Francisco Cruz-Mazo
- Depart. Ingeniería Aeroespacial y Mecánica de Fluidos ETSI, Universidad de Sevilla
- Cyril Danilevski
- European XFEL GmbH
- Connie Darmanin
- Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University
- R. Bruce Doak
- Max Planck Institute for Medical Research
- Martin Domaracky
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Katerina Dörner
- European XFEL GmbH
- Yang Du
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Hans Fangohr
- European XFEL GmbH
- Holger Fleckenstein
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Matthias Frank
- Lawrence Livermore National Laboratory
- Petra Fromme
- School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University
- Alfonso M. Gañán-Calvo
- Depart. Ingeniería Aeroespacial y Mecánica de Fluidos ETSI, Universidad de Sevilla
- Yaroslav Gevorkov
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Klaus Giewekemeyer
- European XFEL GmbH
- Helen Mary Ginn
- Division of Structural Biology, Headington
- Heinz Graafsma
- Deutsches Elektronen-Synchrotron DESY
- Rita Graceffa
- European XFEL GmbH
- Dominic Greiffenberg
- Paul Scherrer Institut
- Lars Gumprecht
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Peter Göttlicher
- Deutsches Elektronen-Synchrotron DESY
- Janos Hajdu
- Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University
- Steffen Hauf
- European XFEL GmbH
- Michael Heymann
- Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry
- Susannah Holmes
- Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University
- Daniel A. Horke
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Mark S. Hunter
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Siegfried Imlau
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Alexander Kaukher
- European XFEL GmbH
- Yoonhee Kim
- European XFEL GmbH
- Alexander Klyuev
- Deutsches Elektronen-Synchrotron DESY
- Juraj Knoška
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Bostjan Kobe
- School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience and Australian Infectious Diseases Research Centre, University of Queensland
- Manuela Kuhn
- Deutsches Elektronen-Synchrotron DESY
- Christopher Kupitz
- Physics Department, University of Wisconsin-Milwaukee
- Jochen Küpper
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Janine Mia Lahey-Rudolph
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Torsten Laurus
- Deutsches Elektronen-Synchrotron DESY
- Karoline Le Cong
- Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, Universität Hamburg
- Romain Letrun
- European XFEL GmbH
- P. Lourdu Xavier
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Luis Maia
- European XFEL GmbH
- Filipe R. N. C. Maia
- Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University
- Valerio Mariani
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Marc Messerschmidt
- European XFEL GmbH
- Markus Metz
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Davide Mezza
- Paul Scherrer Institut
- Thomas Michelat
- European XFEL GmbH
- Grant Mills
- European XFEL GmbH
- Diana C. F. Monteiro
- The Hamburg Center for Ultrafast Imaging, Universität Hamburg
- Andrew Morgan
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Kerstin Mühlig
- Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University
- Anna Munke
- Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University
- Astrid Münnich
- European XFEL GmbH
- Julia Nette
- The Hamburg Center for Ultrafast Imaging, Universität Hamburg
- Keith A. Nugent
- Australian Research Council (ARC) Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Sciences, La Trobe University
- Theresa Nuguid
- Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation, Universität Hamburg
- Allen M. Orville
- Diamond Light Source, Research Complex at Harwell, and University of Oxford, Diamond House, Harwell Science and Innovation Campus
- Suraj Pandey
- Physics Department, University of Wisconsin-Milwaukee
- Gisel Pena
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Pablo Villanueva-Perez
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Jennifer Poehlsen
- Deutsches Elektronen-Synchrotron DESY
- Gianpietro Previtali
- European XFEL GmbH
- Lars Redecke
- Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE)
- Winnie Maria Riekehr
- Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck
- Holger Rohde
- Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE)
- Adam Round
- European XFEL GmbH
- Tatiana Safenreiter
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Iosifina Sarrou
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Tokushi Sato
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Marius Schmidt
- Physics Department, University of Wisconsin-Milwaukee
- Bernd Schmitt
- Paul Scherrer Institut
- Robert Schönherr
- Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lübeck
- Joachim Schulz
- European XFEL GmbH
- Jonas A. Sellberg
- Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology
- M. Marvin Seibert
- Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University
- Carolin Seuring
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Megan L. Shelby
- Lawrence Livermore National Laboratory
- Robert L. Shoeman
- Max Planck Institute for Medical Research
- Marcin Sikorski
- European XFEL GmbH
- Alessandro Silenzi
- European XFEL GmbH
- Claudiu A. Stan
- Physics Department, Rutgers University Newark
- Xintian Shi
- Paul Scherrer Institut
- Stephan Stern
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Jola Sztuk-Dambietz
- European XFEL GmbH
- Janusz Szuba
- European XFEL GmbH
- Aleksandra Tolstikova
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Martin Trebbin
- The Hamburg Center for Ultrafast Imaging, Universität Hamburg
- Ulrich Trunk
- Deutsches Elektronen-Synchrotron DESY
- Patrik Vagovic
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Thomas Ve
- Institute for Glycomics, Griffith University
- Britta Weinhausen
- European XFEL GmbH
- Thomas A. White
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Krzysztof Wrona
- European XFEL GmbH
- Chen Xu
- European XFEL GmbH
- Oleksandr Yefanov
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Nadia Zatsepin
- Department of Physics, Arizona State University
- Jiaguo Zhang
- Paul Scherrer Institut
- Markus Perbandt
- The Hamburg Center for Ultrafast Imaging, Universität Hamburg
- Adrian P. Mancuso
- European XFEL GmbH
- Christian Betzel
- The Hamburg Center for Ultrafast Imaging, Universität Hamburg
- Henry Chapman
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- Anton Barty
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
- DOI
- https://doi.org/10.1038/s41467-018-06156-7
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 11
Abstract
The new European X-Ray Free-Electron Laser (EuXFEL) is the first XFEL that generates X-ray pulses with a megahertz inter-pulse spacing. Here the authors demonstrate that high-quality and damage-free protein structures can be obtained with the currently available 1.1 MHz repetition rate pulses using lysozyme as a test case and furthermore present a β-lactamase structure.