Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic
Mariona Ramos,
Rebeca Martín-García,
M. Ángeles Curto,
Laura Gómez-Delgado,
M. Belén Moreno,
Mamiko Sato,
Elvira Portales,
Masako Osumi,
Sergio A. Rincón,
Pilar Pérez,
Juan C. Ribas,
Juan C.G. Cortés
Affiliations
Mariona Ramos
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain
Rebeca Martín-García
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain
M. Ángeles Curto
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain
Laura Gómez-Delgado
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain
M. Belén Moreno
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain
Mamiko Sato
Laboratory of Electron Microscopy and Bio-imaging Center, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, Japan
Elvira Portales
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain
Masako Osumi
Laboratory of Electron Microscopy and Bio-imaging Center, Japan Women’s University, 2-8-1 Mejirodai, Bunkyo-ku, Tokyo, Japan; Integrated Imaging Research Support (IIRS), Villa Royal Hirakawa 103, 1-7-5 Hirakawa-cho, Chiyoda-ku, Tokyo, Japan
Sergio A. Rincón
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain
Pilar Pérez
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain; Corresponding author
Juan C. Ribas
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain; Corresponding author
Juan C.G. Cortés
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Salamanca, Salamanca, Spain; Corresponding author
Summary: Rod-shaped fission yeast grows through cell wall expansion at poles and septum, synthesized by essential glucan synthases. Bgs1 synthesizes the linear β(1,3)glucan of primary septum at cytokinesis. Linear β(1,3)glucan is also present in the wall poles, suggesting additional Bgs1 roles in growth polarity. Our study reveals an essential collaboration between Bgs1 and Tea1-Tea4, but not other polarity factors, in controlling growth polarity. Simultaneous absence of Bgs1 function and Tea1-Tea4 causes complete loss of growth polarity, spread of other glucan synthases, and spherical cell formation, indicating this defect is specifically due to linear β(1,3)glucan absence. Furthermore, linear β(1,3)glucan absence induces actin patches delocalization and sterols spread, which are ultimately responsible for the growth polarity loss without Tea1-Tea4. This suggests strong similarities in Bgs1 functions controlling actin structures during cytokinesis and polarized growth. Collectively, our findings unveil that cell wall β(1,3)glucan regulates polarized growth, like the equivalent extracellular matrix in neuronal cells.
