EFFECT OF GUNDELIA TOURNEFORTII L. EXTRACT ON LIPID PROFILE AND FLORA BALANCE IN RATS EXPOSED TO EXPERIMENTAL PSEUDOMONAS AERUGINOSA INFECTION

Abstract

The study aimed to investigate the effect of alcoholic Gundelia tourneofortti L. extract on lipid profile and the balance of microbial flora in the intestines of laboratory rats when infected with Pseudomonas aeroginosa. The study was used 20 laboratory rats (Albino Sprague - Dawley) at the age of 7-8 weeks, with an average weights 143-148 gm, divided into four groups, with five replications, and the inhibition activity Pseudomonas aeruginosa was studied by the concentrations of 200, 400 and 600 µg/ml of the extract using the well diffusion assay method. The results found an increase in the alcoholic extraction effectiveness by increasing its concentration and that the alcoholic extraction was the most effective compared with aqueous extract, with the highest inhibition diameter of 30 mm at a concentration of 600 µg/ml. The results also show an increase (P<0.05) in the level of total cholesterol, triglycerides and LDL of groups of rats infected with Pseudo. aeruginosa was 70.5, 73.0, and 14.2 mg/dl, respectively and HDL levels decreased at 27.0 mg/dl, compared with the control group. As for the effect of infection with Pseudo. aeruginosa on the balance of normal flora in rats, it caused a significant decrease at (P<0.05) in the total number of intestinal bacteria, while the total number of LAB bacteria decreased compared to the control group rats.The oral administration of the alcoholic extract at a concentration of 230 and 430 µg/kg with infection has a significant effect in reducing the negative effect of groups infected with Pseudo. aeruginosa on all the above-measured parameter.

Keywords

• alcoholic extract
• gundelia tourneofortti l
• pseudomonas aeroginosa
• lipid profile
• micro flora