Viral-free generation and characterization of a human induced pluripotent stem cell line from dermal fibroblasts

Abstract

Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage.Resource tableUnlabelled TableUnique stem cell line identifierUOWi001-AAlternative name(s) of stem cell lineiPSC RB 9–8InstitutionIllawarra Health and Medical Research Institute, University of WollongongContact information of distributorLezanne Ooi, [email protected] of cell lineiPSCOriginHumanAdditional origin infoAge: 56Sex: femaleCell SourceDermal fibroblastClonalityClonalMethod of reprogrammingTransgene-freeGenetic ModificationNoType of ModificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date21/12/17Cell line repository/bankN/AEthical approvalHE 13/299 University of Wollongong Human Ethics Committee