Three induced pluripotent stem cell lines (TRNDi033-A, TRNDi034-A, TRNDi035-A) generated from lymphoblasts of three apparently healthy individuals

Kofi Owusu-Ansah; Ivan Pavlinov; Miao Xu; Jeanette Beers; Catherine Chen; Wei Zheng; Jizhong Zou

Stem Cell Research (Sep 2023)

Three induced pluripotent stem cell lines (TRNDi033-A, TRNDi034-A, TRNDi035-A) generated from lymphoblasts of three apparently healthy individuals

Kofi Owusu-Ansah,
Ivan Pavlinov,
Miao Xu,
Jeanette Beers,
Catherine Chen,
Wei Zheng,
Jizhong Zou

Affiliations

Kofi Owusu-Ansah: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Ivan Pavlinov: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Miao Xu: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Jeanette Beers: iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Catherine Chen: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA
Wei Zheng: National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA; Corresponding author.
Jizhong Zou: iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

Journal volume & issue: Vol. 71
p. 103135

Abstract

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Expanded human lymphoblast cells from three different aged healthy individuals, 8-year-old male, 0-year-old newborn (NB) male, and 26-year-old female, were used to generate induced pluripotent stem cell (iPSC) lines TRNDi033-A, TRNDi034-A and TRNDi035-A, respectively, by exogenous expression of five reprogramming factors, human OCT4, SOX2, KLF4, L-MYC and LIN28. The authenticity of established iPSC lines was confirmed by the expressions of stem cell markers, karyotype analysis, embryoid body formation, and scorecard analysis. These iPSC lines could serve as healthy donor controls that are age and sex matched for the studies involving patient-specific iPSCs.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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