The impact of three thioxothiazolidin compounds on trehalase activity and development of Spodoptera frugiperda larvae
Yan Wu,
Shangrong Hu,
Qixuan Mao,
Dongmei Shi,
Xiangyu Liu,
Busheng Liu,
Liyuhan Hua,
Gao Hu,
Can Li,
Hongxia Duan,
Bin Tang
Affiliations
Yan Wu
Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Education Department, Department of Biology and Engineering of Environment, Guiyang University, Guiyang, China
Shangrong Hu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
Qixuan Mao
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
Dongmei Shi
Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, China
Xiangyu Liu
Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Education Department, Department of Biology and Engineering of Environment, Guiyang University, Guiyang, China
Busheng Liu
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
Liyuhan Hua
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
Gao Hu
College of Plant Protection, Nanjing Agricultural University, Nanjing, China
Can Li
Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Education Department, Department of Biology and Engineering of Environment, Guiyang University, Guiyang, China
Hongxia Duan
Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, China
Bin Tang
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China
Trehalases (TREs), serving as crucial enzymes regulating trehalose and chitin metabolism in insects, represent prime targets for pest control strategies. We investigated the impact of three thioxothiazolidin compounds (1G, 2G, and 11G) on TRE activity and summarized their effects on the growth and development of Spodoptera frugiperda (Lepidoptera, Noctuidae). The experimental larvae of S. frugiperda were injected with the three thioxothiazolidin compounds (1G, 2G, and 11G), while the control group received an equivalent volume of 2% DMSO as a control. All three compounds had a strong effect on inhibiting TRE activity, significantly prolonging the pre-pupal development stage. However, compared with the 11G-treated group, the survival rate of larvae treated with 1G and 2G was significantly reduced by 31.11% and 27.78% respectively, while the occurrence of phenotypic abnormalities related to growth and development was higher. These results manifest that only the TRE inhibitors, 1G and 2G, modulate trehalose and chitin metabolism pathways of larvae, ultimately resulting in the failure molting and reduction of survival rates. Consequently, the thioxothiazolidin compounds, 1G and 2G, hold potential as environmentally friendly insecticides.
