Survey of medicine susceptibility in Brucella melitensis isolated from raw milk of sheep and goat in Kerman

Abstract

Background and Aim: Brucellosis is one of the most common zoonotic disease between human and cattle breeding. Brucellosis is endemic in all regions of Iran. Since brucella is an arbitrary intracellular organism and a limited range of antibiotics are effective to it, this study is based on the survey of the antibiotic sensitivity to the 9 isolated B.melitensis from raw milk in tribes population. Materials and Methods: Antimicrobial susceptibility of 9 B.melitensis bacteria isolated from nomadic livestock population’s raw milk were studied in the laboratory. The minimum inhibitory concentration (MIC) of tested antimicrobial substances were measured by E- test. To interpret the results, CLSI standards were used for bacteria growth. Results and Conclusions: 9 isolates had a high sensitivity to Doxycycline, two isolates were resistant against three antibiotics Streptomycin (MIC≥240μg/mL) Cotrimoxazole (MIC≥256μg/mL) and Rifampin (MIC≥30μg/mL) and six other isolates were showed Intermediate and sensitivity to former antibiotics. According to results, resistance to Rifampin and Streptomycin and Cotrimoxazole were the important result of this article.

Keywords

• Antibiotic sensitivity
• Brucella melitensis
• Raw milk
• Kerman