Communications Biology (Apr 2021)

Amplification-free RNA detection with CRISPR–Cas13

  • Hajime Shinoda,
  • Yuya Taguchi,
  • Ryoya Nakagawa,
  • Asami Makino,
  • Sae Okazaki,
  • Masahiro Nakano,
  • Yukiko Muramoto,
  • Chiharu Takahashi,
  • Ikuko Takahashi,
  • Jun Ando,
  • Takeshi Noda,
  • Osamu Nureki,
  • Hiroshi Nishimasu,
  • Rikiya Watanabe

DOI
https://doi.org/10.1038/s42003-021-02001-8
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 7

Abstract

Read online

The authors develop a platform (SATORI) that enables accurate and rapid detection of single-stranded RNA at a single-molecule level without a pre-amplification step. As a proof-of-concept, they demonstrate its utility in detecting the SARS-CoV-2 N gene at a minimum concentration of ~5 fM, which is much lower than other amplification-free CRISPR-Cas-based methods.