Analysis of Protein Stability by the Cycloheximide Chase Assay
Shih-Han Kao,
Wen-Lung Wang,
Chi-Yuan Chen,
Yih-Leong Chang,
Yi-Ying Wu,
Yi-Ting Wang,
Shu-Ping Wang,
Alexey Nesvizhskii,
Yu-Ju Chen,
Tse-Ming Hong,
Pan-Chyr Yang
Affiliations
Shih-Han Kao
Research Center for Tumor Medical Science, China Medical University, Taichung, Taiwan
Wen-Lung Wang
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Chi-Yuan Chen
Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, Taoyuan, Taiwan
Yih-Leong Chang
Department of Pathology and Graduate Institute of Pathology, National Taiwan University, Taipei, Taiwan
Yi-Ying Wu
Graduate Institute of Clinical Medicine, National Cheng Kung University, Taipei, Taiwan
Yi-Ting Wang
Chemical Biology and Molecular Biophysics Program, Academia Sinica, Taipei, TaiwanInstitute of Chemistry, Academia Sinica, Taipei, Taiwan, Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan
Shu-Ping Wang
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, USA
Alexey Nesvizhskii
Department of Pathology, University of Michigan, Ann Arbor, USADepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, USA
Yu-Ju Chen
Chemical Biology and Molecular Biophysics Program, Academia Sinica, Taipei, TaiwanInstitute of Chemistry, Academia Sinica, Taipei, Taiwan
Tse-Ming Hong
Graduate Institute of Clinical Medicine, National Cheng Kung University, Taipei, Taiwan
Pan-Chyr Yang
Institute of Biomedical Sciences, Academia Sinica, Taipei, TaiwanDepartment of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan, NTU Center of Genomic Medicine, National Taiwan University, Taipei, Taiwan
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a full cycloheximide chase assay in our laboratory using a lung adenocarcinoma cell line, CL1-5, as a model.
