Ect of Glutathion on resumpsion of Meiosi in-vitro Maturation, and development of immature Mouse oocytes

Abstract

Background and purpose : In this study the effects of glutathion on resumption of meiosis, in-vitro maturation and development of immature mouse oocytes was evaluated.Materials and methods : Immature oocytes recoverd from NMRI mouse strain (4-6 weeks) in four different experimental groups. Group 1 (control group): 345 immature oocytes were matured in MEM-α medium containing FCS 5%, group 1: 338 immature oocytes were cultured in MEM-α medium containing FCS 5% and 1mM glutathione, group 2: 237 immature oocytes curtured in MEM-α medium containing FCS 5%, 7.5 IuhCG, 100mIUrFSH and group 3: 327 immature oocytes were growed in MEM-α medium FCS5%, 7.5 IUhCG, 100mIUrFSH, 1mM glutathione. Fertilization and embryo development were done in T6 medium.Results : There were significant differences between in-vitro maturation rates (group 1: 59.71%, group 2: 79.28%, group 3: 52.74 and group 4: 70.22%) and resumpsion of meiosis (group 1: 74.20%, group 2: 92.88%, group 3: 74.26%, group 4: 86.20%) in different groups.Conclusion : The results of this study showed that glutathione (1mM) enhanced resumpsion of meiosis and in-vitro maturation and development of embryoes.

Keywords

• Glutathion
• Immature oocytes
• Mouse