Sodium dodecyl sulfate-polyacrylamide gel electrophoresis to study photooxidative damage induced by difloxacin to lysozyme

Abstract

The photooxidative damage induced by difloxacin to lysozyme was studied using steady-state illumination. Difloxacin damaged lysozyme structure under 315~375 nm light irradiation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the mechanisms and products of oxidative damage were related to the difloxacin concentration, time of irradiation, and ambient conditions. Longer irradiation time and higher difloxacin concentration lead to more serious damage to lysozyme. When incubated under nitrogen, air, or oxygen, the most serious damage to lysozyme was observed in an oxygen atmosphere. The type I process was examined in a nitrogen-saturated solution, whereas both type I and type II were observed in an aerobic atmosphere, and type II was the dominant process.

Keywords

• lysozyme
• difloxacin
• photooxidative damage
• sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page)