Generation of a PDK-1 knockout human embryonic stem cell line by CRISPR/(WAe009-A-2K) Cas9 editing

Amina Saleem; Mingyu Wei; Muhammad Khawar Abbas; Siyao Zhang; Jiaqi Fan; Yang Xian; Hongfeng Jiang

Stem Cell Research (Mar 2025)

Generation of a PDK-1 knockout human embryonic stem cell line by CRISPR/(WAe009-A-2K) Cas9 editing

Amina Saleem,
Mingyu Wei,
Muhammad Khawar Abbas,
Siyao Zhang,
Jiaqi Fan,
Yang Xian,
Hongfeng Jiang

Affiliations

Amina Saleem: Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing 100029, China
Mingyu Wei: Department of Cardiology, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing 100191, China
Muhammad Khawar Abbas: Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, China; Department of Homoeopathic Medical Sciences, Faculty of Medicine and Allied Health Sciences, The Islamia University of Bahawalpur, 63100 Punjab, Pakistan
Siyao Zhang: Anzhen Hospital, Capital Medical University, Beijing 100029, China; Maternal-Fetal Medicine Centre in Fetal Heart Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
Jiaqi Fan: Anzhen Hospital, Capital Medical University, Beijing 100029, China; Maternal-Fetal Medicine Centre in Fetal Heart Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
Yang Xian: Anzhen Hospital, Capital Medical University, Beijing 100029, China; Maternal-Fetal Medicine Centre in Fetal Heart Disease, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
Hongfeng Jiang: Anzhen Hospital, Capital Medical University, Beijing 100029, China; Beijing Institute of Heart, Lung, and Blood Vessel Diseases, Beijing 100029, China; Corresponding author at: Anzhen Hospital, Capital Medical University, Beijing 100029, China.

Journal volume & issue: Vol. 83
p. 103642

Abstract

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Pyruvate Dehydrogenase Kinase1 (PDK1) belongs to the family of kinases, regulates diverse metabolic processes. PDK1 is a susceptibility locus for heart failure via thinning of ventricle walls, and enlarged atria and ventricles. We successfully developed a PDK1 knockout (PDK1−/−) human embryonic stem cell (hESC) line using an episomal vector-based CRISPR/Cas9 system explore the role of PDK in human heart development. This PDK1-KO hESC line-maintained stem cell-like morphology, pluripotency, and normal karyotype and can differentiate into all three germ layers in vivo. This cell line will be a valuable tool for future research on the role of PDK1 in heart development.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal