Expression of <i>Saccharomyces cerevisiae RER2</i> Gene Encoding <i>Cis</i>-Prenyltransferase in <i>Trichoderma atroviride</i> Increases the Activity of Secretory Hydrolases and Enhances Antimicrobial Features

Abstract

Some Trichoderma spp. exhibit natural abilities to reduce fungal diseases of plants through their mycoparasitic and antagonistic properties. In this study, we created new Trichoderma atroviride strains with elevated antifungal activity. This effect was achieved by improving the activity of cis-prenyltransferase, the main enzyme in dolichol synthesis, by expressing the RER2 gene from Saccharomyces cerevisiae. Since dolichyl phosphate is the carrier of carbohydrate residues during protein glycosylation, activation of its synthesis enhanced the activities of dolichyl-dependent enzymes, DPM synthase and N-acetylglucosamine transferase, as well as stimulated glycosylation of secretory proteins. Cellulases secreted by the transformants revealed significantly higher levels or activities compared to the control strain. Consequently, the resulting Trichoderma strains were more effective against the plant pathogens Pythium ultimum.

Keywords

• <i>cis</i>-prenyltransferase
• glycosylation
• <i>T. atroviride</i>
• antimicrobial activity