Glutation-S-transferase activity of loach embryos during embryogenesis under the action of sodium hypochlorite

Abstract

he effect of sodium hypochlorite obtained at electrolysis of isotonic solution of sodium chloride that is a source of active oxygen on activity of glutation-s-transferase in loach embryos was studied. It was shown that sodium hypochlorite in concentrations 0,5, 1, 2,5, 5, 7,5, 10, 12,5 µg/l caused an increase of glutation-s-transferase activity on the stages of development loach embryos 2, 16, 64, 256, 1024 blastodmeres. On the stage of embryonic development at 64 blastomeres, that corresponds to the sixth dividing of zygote the activity of glutation-s-transferase is maximal and rises, comparative to control, approximatevely on 136% at the action of sodium hypochlorite in all investigated concentrations. While in control samples, the activity of this enzyme is the lowest, comparatively with the stages of development of 2, 16, 256 and 1024 blastomeres. Probably, on this stage of division of embryos, the sodium hypochlorite causes a formation of harmful compounds (for example organic peroxides), that can be inactivated by the glutation-s-transferase. It is found that during incubation of bioblasts in the medium with sodium hypochlorite on the stage of 10th division, the enzymatic activity increased in a dose–dependent maner. Two-factor analysis of variance show that glutation-s-transferase activity depends on the time of development of loach embryos and sodium hypochlorite.

Keywords

• loach embryos
• embryogenesis
• sodium hypochlorite
• glutation-s-transferase