Design of Peptide Ligand for Lactoferrin and Study of Its Binding Specificity
Tatiana Zimina,
Nikita Sitkov,
Vladimir Karasev,
Yury Skorik,
Alexey Kolobov,
Alexander Kolobov,
Nikolay Bunenkov,
Viktor Luchinin
Affiliations
Tatiana Zimina
Department of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, Russia
Nikita Sitkov
Department of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, Russia
Vladimir Karasev
Department of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, Russia
Yury Skorik
Almazov National Medical Research Centre, 197341 Saint Petersburg, Russia
Alexey Kolobov
Centre for Digital Telecommunication Technologies, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, Russia
Alexander Kolobov
Laboratory of Peptide Chemistry, Institute of Human Hygiene, Occupational Pathology and Ecology, 188663 Saint Petersburg, Russia
Nikolay Bunenkov
Department of Bone Marrow Transplantation, Raisa Gorbacheva Research Institute of Children Oncology, Hematology and Transplantation of Pavlov First Saint Petersburg State Medical University, 197022 Saint Petersburg, Russia
Viktor Luchinin
Department of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University “LETI”, 197022 Saint Petersburg, Russia
The in silico modelling of peptides complementary to lactoferrin was carried out using the Protein 3D software package and replication of the natural bonding site between pneumococcal surface protein (PSP) and lactoferrin (LF). The modeling was based on analysis of the conjugated ion–hydrogen bond systems between these proteins (CIHBS). The oligopeptide EEVAPQAQAKIAELENQVHRLE was proposed via computer modelling and synthesized using the solid phase synthesis technique, purified, and analyzed with MS and HPLC methods to confirm >95% purity. The peptide was then studied by capillary electrophoresis (CE). The CE experiments demonstrated the split of peptide zone in the presence of LF, due to complex formation and subsequent mobility change of the system peptide-protein. The reference experiments with homomyeloperoxidase and myoglobin did not show binding with LETI-11.
