Clinical, Cosmetic and Investigational Dermatology (Aug 2022)
Exosomes Derived from Secretome Human Umbilical Vein Endothelial Cells (Exo-HUVEC) Ameliorate the Photo-Aging of Skin Fibroblast
Abstract
Endra Yustin Ellistasari,1 Harijono Kariosentono,2 Bambang Purwanto,3 Brian Wasita,4 Risya Cilmiaty Arief Riswiyant,5 Eti Poncorini Pamungkasari,6 Soetrisno Soetrisno7 1Doctoral Program of Medical Sciences Department, Sebelas Maret University, Surakarta, Indonesia; 2Dermatology and Venereology Department, Sebelas Maret University, Surakarta, Indonesia; 3Internal Medicine Department, Sebelas Maret University, Surakarta, Indonesia; 4Anatomical Pathology Department, Sebelas Maret University, Surakarta, Indonesia; 5Dentistry Department, Sebelas Maret University, Surakarta, Indonesia; 6Public Health Department, Sebelas Maret University, Surakarta, Indonesia; 7Obstetric and Gynecology Department, Sebelas Maret University, Surakarta, IndonesiaCorrespondence: Endra Yustin Ellistasari, Dermatology and Venereology Department, Dr Moewardi General Hospital/Faculty of Medicine Sebelas Maret University, Jl Kol Sutarto 132 Jebres, Surakarta, Central Java, Indonesia, Tel +62 271634634, Email [email protected]: This is an in-vitro experimental study to analyze the effect of Exo-HUVEC on endothelial cell (CD31), cell proliferation, matrix metalloproteinase 1 (MMP-1) and collagen type 1 on irradiated fibroblast with UVB as photo-aging model.Patients and Methods: Fibroblast cultures were divided into 5 groups, namely without UVB exposure, UVB exposure 600mJ/cm2 for 80 seconds as photo-aging model, and UVB exposure +Exo-HUVEC exposure 0.1%, 0.5% and 1%. The endothelial cell was stained with a CD31 marker, MMP-1 were examined with ELISA, cell proliferation is detected using an MTT assay; meanwhile, collagen type 1 deposition and endothelial cell were measured using flowcytometry.Results: This study found positive endothelial cell marker CD31. Significant difference was found in cell proliferation, MMP-1 and collagen type 1 level between the control group with UVB irradiation and the treatment group with Exo-HUVEC (p < 0.05).Conclusion: Exo-HUVEC significantly increases cell proliferation and collagen type 1 level, while decrease MMP-1 levels on irradiated fibroblast; therefore, Exo-HUVEC ameliorate the photo-aging of skin fibroblast.Graphical Abstract: Keywords: photo-aging, Exo-HUVEC, endothelial cell, cell proliferation, MMP-1, collagen type 1