Isolation of <i>Penicillium citrinum</i> from Roots of <i>Clerodendron cyrtophyllum</i> and Application in Biosynthesis of Aglycone Isoflavones from Soybean Waste Fermentation
Duy Tien Doan,
Duc Phuong Luu,
Thanh Duong Nguyen,
Bich Hoang Thi,
Hong Minh Pham Thi,
Huu Nghi Do,
Van Huyen Luu,
The Dan Pham,
Van Thai Than,
Hai Ha Pham Thi,
Minh Quan Pham,
Quoc Toan Tran
Affiliations
Duy Tien Doan
Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Duc Phuong Luu
Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Thanh Duong Nguyen
Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Bich Hoang Thi
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Hong Minh Pham Thi
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Huu Nghi Do
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Van Huyen Luu
Hanoi University of Natural Resources and Environment, Hanoi 10072, Vietnam
The Dan Pham
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Van Thai Than
NTT Institute of High Technology, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam
Hai Ha Pham Thi
Faculty of Biotechnology, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam
Minh Quan Pham
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Quoc Toan Tran
Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 10072, Vietnam
Soybeans offer an abundant source of isoflavones, which confer useful bioactivities when existing in aglycone forms. The conversion of isoflavones into aglycones via fermentation of soybean products is often realized by β-glucosidase, an enzyme produced by fungi. In this study, a filamentous fungus, Clerodendron cyrtophyllum, was isolated from root of Clerodendron cyrtophyllum Turcz, which was able to produce the highest activity of β-glucosidase up to 33.72 U/mL at 144 h during fermentation on Potato Dextrose Broth (PDB). The obtained fungus was grown on isoflavones-rich soybean extract to produce genistein and daidzein, achieving the conversion rate of 98.7%. Genistein and daidzein were isolated and purified by column chromatography using hexane/acetone (29:1/1:1), reaching purities of over 90% of total isoflavones, as identified and determined by TLC, LC-MS/MS, and 1H and 13C NMR spectroscopy. These results imply that the isolated P. citrinum is a potential fungal strain for industrial-scale production of genistein and daidzein from isoflavones-containing soybean extracts. These products may serve as potential raw materials for manufacture of functional foods that are based on aglycones.
