PLoS ONE (Jan 2015)

HIV Drug Resistance Surveillance in Honduras after a Decade of Widespread Antiretroviral Therapy.

  • Santiago Avila-Ríos,
  • Claudia García-Morales,
  • Daniela Tapia-Trejo,
  • Rita I Meza,
  • Sandra M Nuñez,
  • Leda Parham,
  • Norma A Flores,
  • Diana Valladares,
  • Luisa M Pineda,
  • Dixiana Flores,
  • Roxana Motiño,
  • Víctor Umanzor,
  • Candy Carbajal,
  • Wendy Murillo,
  • Ivette Lorenzana,
  • Elsa Y Palou,
  • Gustavo Reyes-Terán

DOI
https://doi.org/10.1371/journal.pone.0142604
Journal volume & issue
Vol. 10, no. 11
p. e0142604

Abstract

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We assessed HIV drug resistance (DR) in individuals failing ART (acquired DR, ADR) and in ART-naïve individuals (pre-ART DR, PDR) in Honduras, after 10 years of widespread availability of ART.365 HIV-infected, ART-naïve, and 381 ART-experienced Honduran individuals were enrolled in 5 reference centres in Tegucigalpa, San Pedro Sula, La Ceiba, and Choluteca between April 2013 and April 2015. Plasma HIV protease-RT sequences were obtained. HIVDR was assessed using the WHO HIVDR mutation list and the Stanford algorithm. Recently infected (RI) individuals were identified using a multi-assay algorithm.PDR to any ARV drug was 11.5% (95% CI 8.4-15.2%). NNRTI PDR prevalence (8.2%) was higher than NRTI (2.2%) and PI (1.9%, p500 vs. <350 CD4+ T cells/μL. PDR in recently infected individuals was 13.6%, showing no significant difference with PDR in individuals with longstanding infection (10.7%). The most prevalent PDR mutations were M46IL (1.4%), T215 revertants (0.5%), and K103NS (5.5%). The overall ADR prevalence in individuals with <48 months on ART was 87.8% and for the ≥48 months on ART group 81.3%. ADR to three drug families increased in individuals with longer time on ART (p = 0.0343). M184V and K103N were the most frequent ADR mutations. PDR mutation frequency correlated with ADR mutation frequency for PI and NNRTI (p<0.01), but not for NRTI. Clusters of viruses were observed suggesting transmission of HIVDR both from ART-experienced to ART-naïve individuals and between ART-naïve individuals.The global PDR prevalence in Honduras remains at the intermediate level, after 10 years of widespread availability of ART. Evidence of ADR influencing the presence of PDR was observed by phylogenetic analyses and ADR/PDR mutation frequency correlations.