Protocol for characterizing strigolactones released by plant roots

Jian You Wang; Guan-Ting Erica Chen; Muhammad Jamil; Justine Braguy; Salim Sioud; Kit Xi Liew; Aparna Balakrishna; Salim Al-Babili

STAR Protocols (Jun 2022)

Protocol for characterizing strigolactones released by plant roots

Jian You Wang,
Guan-Ting Erica Chen,
Muhammad Jamil,
Justine Braguy,
Salim Sioud,
Kit Xi Liew,
Aparna Balakrishna,
Salim Al-Babili

Affiliations

Jian You Wang: The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Corresponding author
Guan-Ting Erica Chen: The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Muhammad Jamil: The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Justine Braguy: The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Salim Sioud: Analytical Chemistry Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Kit Xi Liew: The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Aparna Balakrishna: The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Salim Al-Babili: The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia; Corresponding author

Journal volume & issue: Vol. 3, no. 2
p. 101352

Abstract

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Summary: The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C18 column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination.For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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