Comparative cytogenetics of microsatellite distribution in two tetra fishes Astyanax bimaculatus (Linnaeus, 1758) and Psalidodon scabripinnis (Jenyns, 1842)
Rodrigo Petry Corrêa de Sousa,
Ivanete de Oliveira Furo,
Gláucia Caroline Silva-Oliveira,
Rosigleyse Corrêa de Sousa-Felix,
Carla Denise Bessa-Brito,
Raynara Costa Mello,
Iracilda Sampaio,
Roberto Ferreira Artoni,
Edivaldo Herculano Corrêa de Oliveira,
Marcelo Vallinoto
Affiliations
Rodrigo Petry Corrêa de Sousa
Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil
Ivanete de Oliveira Furo
Universidade Federal Rural da Amazônia, Parauapebas, Pará, Brazil
Gláucia Caroline Silva-Oliveira
Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil
Rosigleyse Corrêa de Sousa-Felix
Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil
Carla Denise Bessa-Brito
Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil
Raynara Costa Mello
Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil
Iracilda Sampaio
Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil
Roberto Ferreira Artoni
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, Paraná, Brazil
Edivaldo Herculano Corrêa de Oliveira
Seção do Meio Ambiente, Instituto Evandro Chagas, Ananindeua, Pará, Brazil
Marcelo Vallinoto
Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança, Pará, Brazil
Background The main cytogenetic studies of the Characidae family comprise the genera Astyanax and Psalidodon involving the use of repetitive DNA probes. However, for the microsatellite classes, studies are still scarce and the function of these sequences in the genome of these individuals is still not understood. Thus, we aimed to analyze and compare the distribution of microsatellite sequences in the species Astyanax bimaculatus and Psalidodon scabripinnis. Methods We collected biopsies from the fins of A. bimaculatus and P. scabripinnis to perform cell culture, followed by chromosome extraction, and mapped the distribution of 14 microsatellites by FISH in both species. Results and Discussion The diploid number observed for both species was 2n = 50, with an acrocentric B microchromosome in A. bimaculatus and a metacentric B chromosome in P. scabripinnis. Regarding FISH, 11 probes hybridized in the karyotype of A. bimaculatus mainly in centromeric regions, and 13 probes hybridized in P. scabripinnis, mainly in telomeric regions, in addition to a large accumulation of microsatellite hybridization on its B chromosome. Conclusion Comparative FISH mapping of 14 microsatellite motifs revealed different patterns of distribution both in autosomes and supernumerary chromosomes of A. bimaculatus and P. scabripinnis, suggesting independent evolutionary processes in each of these species, representing excellent data on chromosome rearrangements and cytotaxonomy.
