Bioactive layered double hydroxide nanoparticles loaded calcein under GelMA scaffolds promoted osteogenesis and angiogenesis for bone regeneration

Yadong Qian; Huiyi Yang; Yuxin Bai; Hongxing Hu; Li Yang; Guangyu Mei; Zhaojie Wang; Rongrong Zhu; Liming Cheng

Materials & Design (Feb 2024)

Bioactive layered double hydroxide nanoparticles loaded calcein under GelMA scaffolds promoted osteogenesis and angiogenesis for bone regeneration

Yadong Qian,
Huiyi Yang,
Yuxin Bai,
Hongxing Hu,
Li Yang,
Guangyu Mei,
Zhaojie Wang,
Rongrong Zhu,
Liming Cheng

Affiliations

Yadong Qian: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China
Huiyi Yang: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China
Yuxin Bai: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China
Hongxing Hu: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China
Li Yang: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China
Guangyu Mei: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China
Zhaojie Wang: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China; Corresponding authors at: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China (R. Zhu, L. Cheng).
Rongrong Zhu: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China; Frontier Science Center for Stem Cell Research, Tongji University, Shanghai 200065, China; Corresponding authors at: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China (R. Zhu, L. Cheng).
Liming Cheng: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China; Clinical Center for Brain and Spinal Cord Research, Tongji University, Shanghai 200065, China; Corresponding authors at: Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Department of Orthopedics, Tongji Hospital affiliated to Tongji University, School of Medicine, School of Life Science and Technology, Tongji University, Shanghai 200065, China (R. Zhu, L. Cheng).

Journal volume & issue: Vol. 238
p. 112621

Abstract

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In regenerative medicine and tissue engineering, the repair and replacement of damaged or lost bone tissue remains an enduring challenge. Traditional approaches often fail to achieve desired outcomes, but recent advancements in material science have introduced innovative solutions to this issue. Gelatin methacryloyl (GelMA), which is derived from gelatin, has a unique combination of biocompatibility and tunable physical properties for bone tissue engineering applications. In this study, a novel composite was constructed to combine the characteristics of GelMA and bioactive layered double hydroxide (LDH) loaded calcein. The GelMA-LDH-calcein (GLC) scaffold is designed to promote the osteogenic differentiation of mesenchymal stem cells (MSCs) and provide a microenvironment conducive to osteogenic differentiation, ultimately increasing the osteogenic activity to support bone regeneration. Our data demonstrated that GLC promoted the osteogenic differentiation related gene (ALP, Runx2, and OPG) expression in bone marrow-derived MSCs (BMSCs) and angiogenesis of human vascular epithelial cells (HUVECs), making it particularly valuable in the application of bone regeneration in vivo. Through the design, synthesis, and optimization of GLC composites, the optimized scaffolds can provide structure supporting and biological activity for cell proliferation, which promoted bone defect regeneration and could be used for further clinical applications.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

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