EBioMedicine (Oct 2015)

The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes

  • Yuan Xie,
  • Tobias Bergström,
  • Yiwen Jiang,
  • Patrik Johansson,
  • Voichita Dana Marinescu,
  • Nanna Lindberg,
  • Anna Segerman,
  • Grzegorz Wicher,
  • Mia Niklasson,
  • Sathishkumar Baskaran,
  • Smitha Sreedharan,
  • Isabelle Everlien,
  • Marianne Kastemar,
  • Annika Hermansson,
  • Lioudmila Elfineh,
  • Sylwia Libard,
  • Eric Charles Holland,
  • Göran Hesselager,
  • Irina Alafuzoff,
  • Bengt Westermark,
  • Sven Nelander,
  • Karin Forsberg-Nilsson,
  • Lene Uhrbom

DOI
https://doi.org/10.1016/j.ebiom.2015.08.026
Journal volume & issue
Vol. 2, no. 10
pp. 1351 – 1363

Abstract

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Glioblastoma (GBM) is the most frequent and malignant form of primary brain tumor. GBM is essentially incurable and its resistance to therapy is attributed to a subpopulation of cells called glioma stem cells (GSCs). To meet the present shortage of relevant GBM cell (GC) lines we developed a library of annotated and validated cell lines derived from surgical samples of GBM patients, maintained under conditions to preserve GSC characteristics. This collection, which we call the Human Glioblastoma Cell Culture (HGCC) resource, consists of a biobank of 48 GC lines and an associated database containing high-resolution molecular data. We demonstrate that the HGCC lines are tumorigenic, harbor genomic lesions characteristic of GBMs, and represent all four transcriptional subtypes. The HGCC panel provides an open resource for in vitro and in vivo modeling of a large part of GBM diversity useful to both basic and translational GBM research.

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