Optimization of technological conditions for preparing corncob reducing sugar by two-enzyme method

Abstract

Objective: This study focuses on realizing the reuse and resource optimization of corncobs. Methods: Using corncob as raw material, using cellulase and hemicellulase to synergistically degrade corncob to prepare reducing sugars. Based on single factor test, response surface methodology was used to determine the mass ratio of cellulase to hemicellulase and double-enzyme process conditions, including addition amount, enzymolysis time, enzymolysis temperature, were optimized. Results: The optimal process parameters for corncob degradation to produce reducing sugars were as follows. The mass ratio of cellulase to hemicellulase was 13∶2, and the amount of double enzyme added was 3.25%, hydrolyzed at 50 ℃ for 5.0 h. The reducing sugar content in the corncob enzymatic hydrolysate prepared under the conditions could reach 12.45 mg/mL. Conclusion: The use of cellulase and hemicellulase to synergize the degradation of corncobs to efficiently and directionally prepare reducing sugars can achieve high-value utilization of corncobs.

Keywords

• corncob
• compound enzyme
• reducing sugar
• response surface