Novel Competitive Fluorescence Sensing Platform for L-carnitine Based on Cationic Pillar[5]Arene Modified Gold Nanoparticles
Xiaoping Tan,
Yang Yang,
Shasha Luo,
Zhong Zhang,
Wenjie Zeng,
Tingying Zhang,
Fawu Su,
Linzong Zhou
Affiliations
Xiaoping Tan
Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, China
Yang Yang
Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, China
Shasha Luo
Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, China
Zhong Zhang
Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, China
Wenjie Zeng
Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, China
Tingying Zhang
Key Lab of Inorganic Special Functional Materials, Chongqing Municipal Education Commission, School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling 408100, China
Fawu Su
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650224, China
Linzong Zhou
School of Geographical Science and Tourism Management, Chuxiong Normal University, Chuxiong 675000, China
Supramolecular host-guest interaction and sensing between cationic pillar[5]arenes (CP5) and L-carnitine were developed by the competitive host-guest recognition for the first time. The fluorescence sensing platform was constructed by CP5 functionalized Au nanoparticles (CP5@Au-NPs) as receptor and probe (rhodamine 123, R123), which shown high sensitivity and selectivity for L-carnitine detection. Due to the negative charge and molecular size properties of L-carnitine, it can be highly captured by the CP5 via electrostatic interactions and hydrophobic interactions. The host-guest mechanism between PP5 and L-carnitine was studied by 1H NMR and molecular docking, indicating that more affinity binding force of CP5 with L-carnitine. Therefore, a selective and sensitive fluorescent method was developed. It has a linear response of 0.1⁻2.0 and 2.0⁻25.0 μM and a detection limit of 0.067 μM (S/N = 3). The fluorescent sensing platform was also used to detect L-carnitine in human serum and milk samples, which provided potential applications for the detection of drugs abuse and had path for guarding a serious food safety issues.
