Characteristics and Mechanisms of Soil Co-Contamination Affecting the Transfer of Cadmium and Arsenic in Peanut (<i>Arachis hypogaea</i> L.)

Abstract

Soil co-contamination with cadmium (Cd) and arsenic (As) occurs frequently and has caused increasing concern. This study aimed to explore the transfer characteristics and the chemical forms, subcellular distribution of Cd and As, as well as the synthesis of phytochelatins (PCs) and other chelates in peanut (Arachis hypogaea L.) plants grown in a Cd and As co-contaminated soil, shedding light on the mechanisms involved. Compared with the single Cd contamination, Cd–As co-contamination led to a higher accumulation of Cd in peanut plants. Conversely, compared to the single As contamination, the As content increased in peanut shoots but decreased in roots and grains under Cd–As co-contamination. Furthermore, the Cd–As interaction resulted in notable changes in peanut plants’ physiological and biochemical responses. In the roots and shoots, there was an 81.8% and 60.0% increase in water-soluble Cd. In the roots, metallothioneins (MTs) content increased by 50%, while PCs increased by 6.4% in the shoots. These changes promoted the translocation of Cd from roots to grains. The Cd–As interaction also influenced the synthesis of MTs in the roots, showing a 41.2% increase, and facilitated the transfer of As to the shoots. In peanut shoots, Cd increased the cell wall fraction of As by 34.5%, decreased the proportion of water-soluble As by 31.8%, and increased PCs content by 6.9%. These changes inhibited the migration of As from shoots to grains. Overall, Cd–As co-contamination increased Cd in peanut grains by increasing water-soluble forms and MTs in roots, while Cd–As co-contamination decreased As in peanut grains by increasing cell wall fractions and PCs in shoots. These findings provide a theoretical basis for understanding Cd–As interactions in soil–peanut systems.

Keywords

• cadmium
• arsenic
• peanut
• subcellular distribution
• chemical forms