Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Jasmine M. Hershewe; Katherine F. Warfel; Shaelyn M. Iyer; Justin A. Peruzzi; Claretta J. Sullivan; Eric W. Roth; Matthew P. DeLisa; Neha P. Kamat; Michael C. Jewett

doi:10.1038/s41467-021-22329-3

Nature Communications (Apr 2021)

Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Jasmine M. Hershewe,
Katherine F. Warfel,
Shaelyn M. Iyer,
Justin A. Peruzzi,
Claretta J. Sullivan,
Eric W. Roth,
Matthew P. DeLisa,
Neha P. Kamat,
Michael C. Jewett

Affiliations

Jasmine M. Hershewe: Department of Chemical and Biological Engineering, Northwestern University, Technological Institute E136
Katherine F. Warfel: Department of Chemical and Biological Engineering, Northwestern University, Technological Institute E136
Shaelyn M. Iyer: Department of Chemical and Biological Engineering, Northwestern University, Technological Institute E136
Justin A. Peruzzi: Department of Chemical and Biological Engineering, Northwestern University, Technological Institute E136
Claretta J. Sullivan: Air Force Research Laboratory, Materials and Manufacturing Directorate, Wright-Patterson Air Force Base
Eric W. Roth: Northwestern University Atomic and Nanoscale Characterization and Experimentation (NUANCE) Center, Tech Institute A/B Wing A173
Matthew P. DeLisa: Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University
Neha P. Kamat: Chemistry of Life Processes Institute, Northwestern University
Michael C. Jewett: Department of Chemical and Biological Engineering, Northwestern University, Technological Institute E136

DOI: https://doi.org/10.1038/s41467-021-22329-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

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Cell-free gene expression systems are an attractive platform for biomanufacturing and synthetic biology. Here the authors characterize native membrane vesicles in E. coli extracts for improved glycoengineering.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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