Biosensors (Jul 2023)

Recent Progress of Activity-Based Fluorescent Probes for Imaging Leucine Aminopeptidase

  • Ze-Jun Li,
  • Cai-Yun Wang,
  • Liang Xu,
  • Zhen-Yu Zhang,
  • Ying-Hao Tang,
  • Tian-Yi Qin,
  • Ya-Long Wang

DOI
https://doi.org/10.3390/bios13070752
Journal volume & issue
Vol. 13, no. 7
p. 752

Abstract

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Leucine aminopeptidase (LAP) is an important protease that can specifically hydrolyze Leucine residues. LAP occurs in microorganisms, plants, animals, and humans and is involved in a variety of physiological processes in the human body. In the physiological system, abnormal levels of LAP are associated with a variety of diseases and pathological processes, such as cancer and drug-induced liver injury; thus, LAP was chosen as the early biochemical marker for many physiological processes, including cancer. Considering the importance of LAP in physiological and pathological processes, it is critical that high-efficiency and dependable technology be developed to monitor LAP levels. Herein, we summarize the organic small molecule fluorescence/chemiluminescence probes used for LAP detection in recent years, which can image LAP in cancer, drug-induced liver injury (DILI), and bacteria. It can also reveal the role of LAP in tumors and differentiate the serum of cirrhotic, drug-induced liver injury and normal models.

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