Novel <i>Acinetobacter baumannii</i> Myovirus TaPaz Encoding Two Tailspike Depolymerases: Characterization and Host-Recognition Strategy
Anastasia S. Shchurova,
Mikhail M. Shneider,
Nikolay P. Arbatsky,
Alexander S. Shashkov,
Alexander O. Chizhov,
Yuriy P. Skryabin,
Yulia V. Mikhaylova,
Olga S. Sokolova,
Andrey A. Shelenkov,
Konstantin A. Miroshnikov,
Yuriy A. Knirel,
Anastasia V. Popova
Affiliations
Anastasia S. Shchurova
Moscow Institute of Physics and Technology (National Research University), Institutskiy per. 9, Dolgoprudny, 141700 Moscow Region, Russia
Mikhail M. Shneider
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Nikolay P. Arbatsky
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
Alexander S. Shashkov
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
Alexander O. Chizhov
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
Yuriy P. Skryabin
State Research Center for Applied Microbiology and Biotechnology, 24 “Quarter A” Territory, Obolensk, City District Serpukhov, 142279 Moscow Region, Russia
Yulia V. Mikhaylova
Central Scientific Research Institute of Epidemiology, Novogireevskaya 3a, 111123 Moscow, Russia
Olga S. Sokolova
Biology Department, Lomonosov Moscow State University, Leninskie Gory 1, 119234 Moscow, Russia
Andrey A. Shelenkov
Central Scientific Research Institute of Epidemiology, Novogireevskaya 3a, 111123 Moscow, Russia
Konstantin A. Miroshnikov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
Yuriy A. Knirel
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
Anastasia V. Popova
State Research Center for Applied Microbiology and Biotechnology, 24 “Quarter A” Territory, Obolensk, City District Serpukhov, 142279 Moscow Region, Russia
Acinetobacter baumannii, one of the most significant nosocomial pathogens, is capable of producing structurally diverse capsular polysaccharides (CPSs) which are the primary receptors for A. baumannii bacteriophages encoding polysaccharide-degrading enzymes. To date, bacterial viruses specifically infecting A. baumannii strains belonging to more than ten various capsular types (K types) were isolated and characterized. In the present study, we investigate the biological properties, genomic organization, and virus–bacterial host interaction strategy of novel myovirus TaPaz isolated on the bacterial lawn of A. baumannii strain with a K47 capsular polysaccharide structure. The phage linear double-stranded DNA genome of 93,703 bp contains 178 open reading frames. Genes encoding two different tailspike depolymerases (TSDs) were identified in the phage genome. Recombinant TSDs were purified and tested against the collection of A. baumannii strains belonging to 56 different K types. One of the TSDs was demonstrated to be a specific glycosidase that cleaves the K47 CPS by the hydrolytic mechanism.
