Viruses (May 2021)

Novel <i>Acinetobacter baumannii</i> Myovirus TaPaz Encoding Two Tailspike Depolymerases: Characterization and Host-Recognition Strategy

  • Anastasia S. Shchurova,
  • Mikhail M. Shneider,
  • Nikolay P. Arbatsky,
  • Alexander S. Shashkov,
  • Alexander O. Chizhov,
  • Yuriy P. Skryabin,
  • Yulia V. Mikhaylova,
  • Olga S. Sokolova,
  • Andrey A. Shelenkov,
  • Konstantin A. Miroshnikov,
  • Yuriy A. Knirel,
  • Anastasia V. Popova

DOI
https://doi.org/10.3390/v13060978
Journal volume & issue
Vol. 13, no. 6
p. 978

Abstract

Read online

Acinetobacter baumannii, one of the most significant nosocomial pathogens, is capable of producing structurally diverse capsular polysaccharides (CPSs) which are the primary receptors for A. baumannii bacteriophages encoding polysaccharide-degrading enzymes. To date, bacterial viruses specifically infecting A. baumannii strains belonging to more than ten various capsular types (K types) were isolated and characterized. In the present study, we investigate the biological properties, genomic organization, and virus–bacterial host interaction strategy of novel myovirus TaPaz isolated on the bacterial lawn of A. baumannii strain with a K47 capsular polysaccharide structure. The phage linear double-stranded DNA genome of 93,703 bp contains 178 open reading frames. Genes encoding two different tailspike depolymerases (TSDs) were identified in the phage genome. Recombinant TSDs were purified and tested against the collection of A. baumannii strains belonging to 56 different K types. One of the TSDs was demonstrated to be a specific glycosidase that cleaves the K47 CPS by the hydrolytic mechanism.

Keywords