Nature Communications (May 2019)
Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs
- Kale Kundert,
- James E. Lucas,
- Kyle E. Watters,
- Christof Fellmann,
- Andrew H. Ng,
- Benjamin M. Heineike,
- Christina M. Fitzsimmons,
- Benjamin L. Oakes,
- Jiuxin Qu,
- Neha Prasad,
- Oren S. Rosenberg,
- David F. Savage,
- Hana El-Samad,
- Jennifer A. Doudna,
- Tanja Kortemme
Affiliations
- Kale Kundert
- Graduate Group in Biophysics, University of California San Francisco
- James E. Lucas
- UC Berkeley – UCSF Graduate Program in Bioengineering, University of California San Francisco
- Kyle E. Watters
- Department of Molecular and Cell Biology, University of California Berkeley
- Christof Fellmann
- Department of Molecular and Cell Biology, University of California Berkeley
- Andrew H. Ng
- UC Berkeley – UCSF Graduate Program in Bioengineering, University of California San Francisco
- Benjamin M. Heineike
- Bioinformatics Graduate Program, University of California San Francisco
- Christina M. Fitzsimmons
- Chemistry and Chemical Biology Graduate Program, University of California San Francisco
- Benjamin L. Oakes
- Department of Molecular and Cell Biology, University of California Berkeley
- Jiuxin Qu
- Department of Medicine, University of California San Francisco
- Neha Prasad
- Department of Medicine, University of California San Francisco
- Oren S. Rosenberg
- Department of Medicine, University of California San Francisco
- David F. Savage
- Department of Molecular and Cell Biology, University of California Berkeley
- Hana El-Samad
- Chan Zuckerberg Biohub
- Jennifer A. Doudna
- Department of Molecular and Cell Biology, University of California Berkeley
- Tanja Kortemme
- Chan Zuckerberg Biohub
- DOI
- https://doi.org/10.1038/s41467-019-09985-2
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 11
Abstract
Control of CRISPR-Cas9 activity allows for fine-tuning of editing and gene expression. Here the authors use gRNAs modified with RNA aptamers to enable small molecule control in bacterial systems.
