Developing new approaches to hyperlipidemia correction taking into account changes in fatty acids structure of blood serum

Abstract

It is still a pressing issue in contemporary medicine to examine pathogenesis mechanisms and update procedures aimed at treating atherosclerosis. Developments by domestic and foreign researchers revealed that complex molecular and cellular studies on a mechanism of impacts exerted by vegetative-based medications, produced both domestically and abroad and used to treat atherosclerosis, were of primary importance in practical medicine in terms of educing population health risks. It is assumed that disorders in formation and transfer of non-esterified fatty acids (NEFA) in blood plasma are a major reason for hypertriglyceridemia occurrence. The article contains research data on lipid metabolism parameters taken in dynamics of experimental hypercholesterolemia development. Performed research allowed revealing hypolipidemic effects produced by a biologically active additive called Biomays. We developed theoretical grounds for recommendations that should be given to patients suffering from hyperlipidemia and not getting proper therapeutic effects form treatment with statins. We recommend a complex approach which includes a BAA (biologically active additive) Biomays made of dried wheat sprouts in order to reduce risks caused by complications related to treatment with statins. Our research goal was to develop new approaches to correcting hyperlipidemia basing on changes in fatty acids structure of blood serum. The experiments were performed on 30 male rabbits belonging to chinchilla breed with initial body mass equal to 2,500–3,00 grams; animals were divided into 5 groups, 6 animals in each, depending on a research goal and treatment procedures. We started a 30-day treatment of experimental animals with ultrox and Biomays in doses equal to 0.6 mg/kg and 142 mg/kg accordingly after they had been given cholesterol for 2 months. We determined fatty acids structure of blood serum with a triple quadrupole chromato-mass-spectrometer with gas chromatographer (GC-MS/MS) TRACE 1310 TSQ 8000 and automated autosampler CTC TriPlus RSH produced by Thermo Fisher Scientific (the USA).Combined application of ultrox and Biomays led to more significant hypolipidemic effects. Use of statins and wheat sprouts had a distinct positive effect on contents of saturated and poly-unsaturated fatty acids n blood such as linoleic acid and linolenic acid.

Keywords

• fatty acids
• water-soluble vitamins
• policosanol
• biomays biologically active additive
• mass-spectrometry
• gas chromatography
• hyperlipidemia