Frontiers in Immunology (Aug 2022)

Exploration of PDCoV-induced apoptosis through mitochondrial dynamics imbalance and the antagonistic effect of SeNPs

  • Zhihua Ren,
  • Zhihua Ren,
  • Yueru Yu,
  • Xiaojie Zhang,
  • Qiuxiang Wang,
  • Junliang Deng,
  • Chaoxi Chen,
  • Riyi Shi,
  • Zhanyong Wei,
  • Hui Hu

DOI
https://doi.org/10.3389/fimmu.2022.972499
Journal volume & issue
Vol. 13

Abstract

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Porcine Deltacoronavirus (PDCoV), an enveloped positive-strand RNA virus that causes respiratory and gastrointestinal diseases, is widely spread worldwide, but there is no effective drug or vaccine against it. This study investigated the optimal Selenium Nano-Particles (SeNPs) addition concentration (2 - 10 μg/mL) and the mechanism of PDCoV effect on ST (Swine Testis) cell apoptosis, the antagonistic effect of SeNPs on PDCoV. The results indicated that 4 μg/mL SeNPs significantly decreased PDCoV replication on ST cells. SeNPs relieved PDCoV-induced mitochondrial division and antagonized PDCoV-induced apoptosis via decreasing Cyt C release and Caspase 9 and Caspase 3 activation. The above results provided an idea and experimental basis associated with anti-PDCoV drug development and clinical use.

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