Cytotoxicity of magnetite nanoparticles deposited in sodium chloride matrix and their functionalized analogues in erythrocytes
Stanislav Lytvyn,
Elena Vazhnichaya,
Yurii Kurapov,
Oleksandr Semaka,
Lyubov Babijchuk,
Pavlo Zubov
Affiliations
Stanislav Lytvyn
Laboratory of Electron Beam Nanotechnology of Inorganic Materials for Medicine, E. O. Paton Electric Welding Institute of the National Academy of Sciences of Ukraine, 11, Kazymyr Malevych Street, Kyiv, 03150, Ukraine; Corresponding author: Stanislav Lytvyn Tel: +38 (044) 205 2286 Fax: +38 (044) 200 6855
Elena Vazhnichaya
Department of Pharmacology, Clinical Pharmacology and Pharmacy, Poltava State Medical University, 23, Shevchenko Street, Poltava, 36011, Ukraine
Yurii Kurapov
Laboratory of Electron Beam Nanotechnology of Inorganic Materials for Medicine, E. O. Paton Electric Welding Institute of the National Academy of Sciences of Ukraine, 11, Kazymyr Malevych Street, Kyiv, 03150, Ukraine
Oleksandr Semaka
Department of Pharmacology, Clinical Pharmacology and Pharmacy, Poltava State Medical University, 23, Shevchenko Street, Poltava, 36011, Ukraine
Lyubov Babijchuk
Department of Cryocytology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, 23, Pereyaslavska Street, Kharkiv, 61016, Ukraine
Pavlo Zubov
Department of Cryocytology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, 23, Pereyaslavska Street, Kharkiv, 61016, Ukraine
The synthesis of covered nanoparticles provides new properties to the materials for biomedical applications. This fully applies to iron oxide nanoparticles. The research aim was to study features of the magnetite nanoparticles synthesized by electron beam technology as well as to investigate their functionalization and cytotoxicity. Nanoparticle characteristics were determined by standard methods. Cytotoxiciy of nanoparticles was studied using erythrocyte model. It was shown that the original magnetite nanoparticles in the sodium chloride matrix can be functionalized with polyvinylpyrrolidone and ethylmethylhydroxypyridine succinate, an antioxidant. All investigated nanoparticles were non-toxic for erythrocytes at concentrations up to 100 μg Fe/ml. At 100-200 μg Fe/ml, they increased the amount of cells expressing phosphatidylserine on the outer membrane, the count of pathological forms of erythrocytes and hemolysis. These phenomena were less pronounced if the nanosystem included the antioxidant. Therefore, magnetite nanoparticles can be obtained by electron beam technology and functionalized to form non-toxic nanosystems.
