Songklanakarin Journal of Science and Technology (SJST) (Jul 2006)

Cultivar, explant type and culture medium influencing embryogenesis and organogenegenesis in Anthurium spp.

  • Yaowaphan Sontikun,
  • Thanyaporn Susanon,
  • Sompong Te-chato

Journal volume & issue
Vol. 28, no. 4
pp. 717 – 722

Abstract

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The effect of cultivars, culture media, explant type and wounding on culture response of anthurium were studied. Valantino gave the highest callus formation (83.73%) significantly different from Sonat (78.73%) and Plew Thien Phuket (45.66%). Valantino and Plew Thien Phuket gave meristematic nodular callus (MNC) whereas Sonat produced embryogenic-like callus (ELC). Modified Murashige and Skoog (MMS) medium gave the highest callus formation from both leaf (86.6%) and node (100%). Callus obtained in Nitsch and Nitsch (NN) and MMS was MNC while woody plant (WPM) medium provided ELC. For explant types, internode gave the highest callus formation (72.63%). Nodal and internodal explant gave ELC while the leaf explant yielded MNC. Wounding leaf blades tended to promote more MNC.

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